{"id":8669,"date":"2023-04-18T14:22:34","date_gmt":"2023-04-18T17:22:34","guid":{"rendered":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/?page_id=8669"},"modified":"2023-04-18T14:22:34","modified_gmt":"2023-04-18T17:22:34","slug":"cesar-bertagia-pasqualetti-dr","status":"publish","type":"page","link":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/cesar-bertagia-pasqualetti-dr\/","title":{"rendered":"Cesar Bertagia Pasqualetti &#8211; DR"},"content":{"rendered":"<h2 style=\"text-align: center\"><a href=\"arquivos.ambiente.sp.gov.br\/pgibt\/2015\/02\/faixapos6.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-3777 size-full\" style=\"border: 0px\" src=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/2015\/02\/faixapos6.jpg\" alt=\"faixapos6\" width=\"950\" height=\"163\" srcset=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/2015\/02\/faixapos6.jpg 950w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/2015\/02\/faixapos6-768x131.jpg 768w\" sizes=\"(max-width: 950px) 100vw, 950px\" \/><\/a><\/h2>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<h2 style=\"text-align: center\">Cesar Bertagia Pasqualetti<\/h2>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<p style=\"text-align: center\">No dia 29 de julho de 2019, o aluno Cesar Bertagia Pasqualetti, bolsista FAPESP, do Programa de P\u00f3s Gradua\u00e7\u00e3o em Biodiversidade Vegetal e<br \/>\nMeio Ambiente, defendeu sua tese de doutorado intitulada<br \/>\n\u201cVaria\u00e7\u00f5es dos metab\u00f3litos prim\u00e1rios de esp\u00e9cies de <em>Rhodophyta<\/em> das regi\u00f5es Ant\u00e1rtica e subant\u00e1rtica\u201d.<\/p>\n<p style=\"text-align: center\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-full wp-image-8670\" src=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2023\/04\/cesar_bertagia_pasqualetti_dr.jpg\" alt=\"\" width=\"1000\" height=\"750\" srcset=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2023\/04\/cesar_bertagia_pasqualetti_dr.jpg 1000w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2023\/04\/cesar_bertagia_pasqualetti_dr-256x192.jpg 256w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2023\/04\/cesar_bertagia_pasqualetti_dr-960x720.jpg 960w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2023\/04\/cesar_bertagia_pasqualetti_dr-768x576.jpg 768w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2023\/04\/cesar_bertagia_pasqualetti_dr-250x188.jpg 250w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2023\/04\/cesar_bertagia_pasqualetti_dr-550x413.jpg 550w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2023\/04\/cesar_bertagia_pasqualetti_dr-800x600.jpg 800w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2023\/04\/cesar_bertagia_pasqualetti_dr-240x180.jpg 240w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2023\/04\/cesar_bertagia_pasqualetti_dr-400x300.jpg 400w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2023\/04\/cesar_bertagia_pasqualetti_dr-667x500.jpg 667w\" sizes=\"(max-width: 1000px) 100vw, 1000px\" \/>A banca examinadora foi composta pela orientadora Prof. Dra. Nair Sumie Yokoya (N\u00facleo de Pesquisa em Ficologia do Instituto de Bot\u00e2nica de S\u00e3o Paulo), e pelos Prof. Dra. Marcia Graminha (), Prof. Dra. Aline Paternostro Martins (Universidade S\u00e3o Camilo), Prof. Dr. Danilo Centeno (Universidade Federal do ABC) e a Prof. Dra. Mutue Toyota Fuji (N\u00facleo de Pesquisa em Ficologia do Instituto de Bot\u00e2nica de S\u00e3o Paulo).<\/p>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<h3 style=\"text-align: center\">Varia\u00e7\u00f5es dos metab\u00f3litos prim\u00e1rios de esp\u00e9cies de <em>Rhodophyta<\/em> das regi\u00f5es Ant\u00e1rtica e subant\u00e1rtica<\/h3>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<p style=\"text-align: center\"><strong>ABSTRACT<\/strong><\/p>\n<p style=\"text-align: justify\">The seasons in the polar regions are very singular, with long periods of low light due to polar night and ice cover during winter, and long periods of light interspersed with a few dark hours during summer. To survive such conditions, benthic marine macroalgae needs a strategy of biochemical and physiological adaptation, ensuring protection and energy in the absence of light, making them resistant to wave action, freezing \/ thawing cycle, desiccation and salinity variations. Therefore, the present study aimed to evaluate the involvement of metabolites (pigments, soluble proteins and carbohydrates) in different survival strategies adopted by macroalgae species from the Antarctic and subantarctic region, collected at different latitudes and life history phases. For this, the studied species were <em>Gigartina skottsbergii<\/em> Setchell &amp; N.L. Gardner, <em>Curdiea racovtizae<\/em> Hariot, <em>Iridaea cordata<\/em> (Turner) Bory de Saint-Vincent, <em>Palmaria decipiens<\/em> (Reinsch) R.W. Ricker, <em>Georgiella confluens<\/em> (Reinsch) Kylin, and <em>Plocamium cartilagineum<\/em> (Linnaeus) P.S.Dixon. Samples were collected in 14 sites at different latitudes in the antarctic regions. <em>Gi. skottsbergii<\/em> were collected in 4 sites in the Magellan region (subantarctic region), beyond the Antarctic regions collecting sites. The metabolites varied according to the collecting sites, species and life history phase. The floridean starch plays an important role in reproductive phases, since fertile thallus of <em>Gi. skottsbergii, I. cordata <\/em>and<em> C. racovitzae<\/em> presented higher concentrations of floridean starch than non-fertile thallus. In addition, this storage carbohydrate was higher in <em>Gi. skottsbergii<\/em> from the Antarctic region than subantarctic region, showing a relation to the abiotic conditions of each collecting sites. Non-fertile thallus of <em>Gi. skottsbergii, I. cordata <\/em>and<em> C. racovitzae<\/em> had higher concentrations of photosynthetic pigments, showing a pigment investment, which is probably related to thallus growth. The low concentrations of photosynthetic pigments, mainly carotenoids, in the carrageenophytes <em>Gi. skottsbergii<\/em> and <em>I. cordata<\/em> may be related to the types of cell wall polysaccharides that these algae synthesize and the position that these species occupy in the rocky shore, when compared to the other species. The sulfation degree of polysaccharides has also been shown to vary among species and life history phases of <em>Gi. skottsbergii, I. cordata <\/em>and<em> C. racovitzae.<\/em> The highest concentrations of LMWC in <em>C. racovitzae<\/em> and <em>Ge. confluens<\/em> and lower concentrations in <em>Pa. decipiens<\/em> show that carbohydrate metabolism is an important factor in the different survival strategies of these organisms. In addition, as like polysaccharides, LMWC are influenced by the life history phases and the environmental conditions imposed by the different latitudes, mainly by salinity and temperature. The method of identifying LMWC in red macroalgae by HPAEC \/ PAD in column CarboPAC MA1 was efficient for identification of physiological and economical importance carbohydrates, such as polyols. Through it, the glycerol and floridoside were the only carbohydrates presented in all samples. On the other hand, the mannitol and trehalose were present only in species from deeper regions on the rocky shore, and these may be associated with the vertical distribution of the species. The presence of mannitol in <em>Pa. decipiens<\/em> sample from BR, suggests that this polyol may offer protection against freezing, since this is the place with the lowest temperature. Therefore, we can conclude that non-fertile thallus prioritize energy investment in photosynthetic pigments, since there are no reproductive structures. On the other hand, the higher concentration of floridean starch in fertile thallus and in low latitudes sites, shows the importance of this storage product in reproductive phases and at lower temperatures. The high concentrations of LMWC in cold regions show a protective role against low temperatures, since all species presented glycerol and floridoside, which are able to decrease the freezing point. Thus, we can affirm that carbohydrate metabolism is a determining factor in the survival strategies of these macroalgae in extreme environments.<\/p>\n<p style=\"text-align: justify\"><strong>Key-words:<\/strong> Macroalgae, Rhodophyta, Antarctica, subantarctic region, pigments, proteins, carbohydrates, LMWC<\/p>\n<p style=\"text-align: center\"><strong>RESUMO<\/strong><\/p>\n<p style=\"text-align: justify\">As esta\u00e7\u00f5es do ano nas regi\u00f5es polares s\u00e3o bem caracter\u00edsticas, com longos per\u00edodos de baixa luminosidade devido \u00e0 noite polar e cobertura de gelo durante o inverno, e longos per\u00edodos de luz intercalados por poucas horas de escuro durante o ver\u00e3o. Para sobreviver a tais condi\u00e7\u00f5es, as algas marinhas bent\u00f4nicas necessitam de uma estrat\u00e9gia de adapta\u00e7\u00e3o bioqu\u00edmica e fisiol\u00f3gica, garantindo prote\u00e7\u00e3o e energia na aus\u00eancia de luz, tornando-as resistentes \u00e0 a\u00e7\u00e3o das ondas, ao congelamento, \u00e0 desseca\u00e7\u00e3o e \u00e0s varia\u00e7\u00f5es de salinidade. Portanto, o presente trabalho teve como principal objetivo avaliar o envolvimento dos metab\u00f3litos (pigmentos, prote\u00ednas sol\u00faveis e carboidratos) em diferentes estrat\u00e9gias de sobreviv\u00eancia adotadas pelas esp\u00e9cies de macroalgas da regi\u00e3o ant\u00e1rtica e subant\u00e1rtica, coletadas em diferentes latitudes e hist\u00f3rico de vida. Para isso, as esp\u00e9cies estudadas foram <em>Gigartina skottsbergii<\/em> Setchell &amp; N.L. Gardner, <em>Curdiea racovtizae<\/em> Hariot, <em>Iridaea cordata<\/em> (Turner) Bory de Saint-Vincent, <em>Palmaria decipiens<\/em> (Reinsch) R.W. Ricker, <em>Georgiella confluens<\/em> (Reinsch) Kylin, e <em>Plocamium cartilagineum<\/em> (Linnaeus) P.S.Dixon. As amostras foram coletadas em 14 locais diferentes ao longo das Ilhas Shetland do Sul e Peninsula Ant\u00e1rtica. <em>Gi. skottsbergii<\/em> foi coletada em 4 locais da regi\u00e3o de Magalh\u00e3es, no Chile (regi\u00e3o subant\u00e1rtica), al\u00e9m dos locais da regi\u00e3o Ant\u00e1rtica. Os metab\u00f3litos analisados variaram conforme o local de coleta, a esp\u00e9cie e a fase do hist\u00f3rico de vida. Foi poss\u00edvel notar que o amido das flor\u00eddeas tem um papel importante na reprodu\u00e7\u00e3o, j\u00e1 que talos f\u00e9rteis de <em>Gi. skottsbergii, I. cordata e C. racovitzae <\/em>apresentaram maiores concentra\u00e7\u00f5es de amido das flor\u00eddeas do que talos n\u00e3o f\u00e9rteis. Al\u00e9m disso, a concentra\u00e7\u00e3o deste produto de reserva foi maior em<em> Gi. skottsbergii<\/em> de regi\u00e3o ant\u00e1rtica do que em regi\u00e3o subant\u00e1rtica, mostrando uma rela\u00e7\u00e3o deste composto com as condi\u00e7\u00f5es abi\u00f3ticas de cada local. J\u00e1 os talos n\u00e3o f\u00e9rteis de <em>Gi. skottsbergii, I. cordata e C. racovitzae<\/em> apresentaram maiores concentra\u00e7\u00f5es de pigmentos fotossintetizantes, mostrando que o investimento em pigmentos est\u00e1 provavelmente relacionado ao crescimento do talo. As baixas concentra\u00e7\u00f5es de pigmentos fotossintetizantes, principalmente carotenoides, nas carragen\u00f3fitas <em>Gi. skottsbergii<\/em> e <em>I. cordata<\/em>, podem estar relacionadas aos tipos de polissacar\u00eddeos de parede celular que essas algas sintetizam e a posi\u00e7\u00e3o que estas esp\u00e9cies ocupam no cost\u00e3o rochoso, se comparada as demais esp\u00e9cies estudadas. O grau de sulfata\u00e7\u00e3o dos polissacar\u00eddeos tamb\u00e9m variou entre as esp\u00e9cies, e entre as fases do hist\u00f3rico de vida de <em>Gi. skottsbergii<\/em>, <em>I. cordata <\/em>e<em> C. racovitzae.<\/em> As maiores concentra\u00e7\u00f5es de CBPM encontradas em <em>C. racovitzae<\/em> e <em>Ge. confluens<\/em> e menores concentra\u00e7\u00f5es em <em>Pa. decipiens<\/em> revelam que o metabolismo de carboidratos \u00e9 um fator importante nas diferentes estrat\u00e9gias de sobreviv\u00eancia desses organismos. Al\u00e9m disso, assim como os polissacar\u00eddeos, os CBPM s\u00e3o influenciados pelas fases do hist\u00f3rico de vida das esp\u00e9cies e pelas condi\u00e7\u00f5es ambientais impostas pelas diferentes latitudes, principalmente por salinidade e temperatura. O m\u00e9todo de identifica\u00e7\u00e3o de CBPM em macroalgas vermelhas por HPAEC\/PAD em coluna CarboPAC MA1 se mostrou eficiente para identifica\u00e7\u00e3o de compostos de grande import\u00e2ncia fisiol\u00f3gica e econ\u00f4mica, como os poli\u00f3is. Atrav\u00e9s dele, foi poss\u00edvel notar que o glicerol e o floridos\u00eddeo foram os \u00fanicos carboidratos presentes em todas as amostras. Por outro lado, o manitol e a trealose foram carboidratos presentes apenas em esp\u00e9cies que ocorrem em regi\u00f5es mais profundas do cost\u00e3o rochoso, podendo estar associados a distribui\u00e7\u00e3o vertical das esp\u00e9cies. A presen\u00e7a de manitol em amostra de <em>Pa. decipiens<\/em> da esta\u00e7\u00e3o Brown, sugere que este poliol pode oferecer prote\u00e7\u00e3o contra congelamento, j\u00e1 que \u00e9 o local com a menor temperatura. Com os resultados obtidos neste trabalho, \u00e9 poss\u00edvel concluir que as talos n\u00e3o-f\u00e9rteis priorizam o investimento de energia em pigmentos fotossintetizantes, j\u00e1 que n\u00e3o h\u00e1 estruturas reprodutivas. Por outro lado, a maior concentra\u00e7\u00e3o de amido das flor\u00eddeas em talos f\u00e9rteis e de menores latitudes, mostra a import\u00e2ncia deste produto de reserva em per\u00edodos reprodutivos e em temperaturas mais baixas. As altas concentra\u00e7\u00f5es de CBPM em regi\u00f5es mais frias revelam um papel de prote\u00e7\u00e3o contra baixas temperaturas, j\u00e1 que todas as esp\u00e9cies apresentaram glicerol e floridosideo, que s\u00e3o capazes de diminuir o ponto de congelamento. Assim, podemos afirmar que o metabolismo de carboidratos \u00e9 um fator determinante nas estrat\u00e9gias de sobreviv\u00eancia destas macroalgas em ambientes extremos.<\/p>\n<p style=\"text-align: justify\"><strong>Palavras-chave:<\/strong> Macroalgas, Rhodophyta, Ant\u00e1rtica, regi\u00e3o subant\u00e1rtica, pigmentos, prote\u00ednas, carboidratos, CBPM<\/p>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<p style=\"text-align: center\"><a href=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2023\/04\/cesar_bertagia_pasqualetti_dr.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter\" src=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/2013\/09\/pdf_grande.gif\" alt=\"pdf_grande\" width=\"60\" height=\"60\" \/><\/a><a href=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2023\/04\/cesar_bertagia_pasqualetti_dr.pdf\"><strong> Cesar Bertagia Pasqualetti<\/strong><\/a><br \/>\n<a href=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2023\/04\/cesar_bertagia_pasqualetti_dr.pdf\">Varia\u00e7\u00f5es dos metab\u00f3litos prim\u00e1rios de esp\u00e9cies de <em>Rhodophyta<\/em> das regi\u00f5es Ant\u00e1rtica e subant\u00e1rtica<\/a><\/p>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<p style=\"text-align: center\"><strong>\u00a0<a href=\"www.infraestruturameioambiente.sp.gov.br\/pgibt\/dissertacoesteses\/\">VOLTAR AS DISSERTA\u00c7\u00d5ES E TESES<\/a><\/strong><\/p>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n","protected":false},"excerpt":{"rendered":"<p>Cesar Bertagia Pasqualetti No dia 29 de julho de 2019, o aluno Cesar Bertagia Pasqualetti, bolsista FAPESP, do Programa de P\u00f3s Gradua\u00e7\u00e3o em Biodiversidade Vegetal e Meio Ambiente, defendeu sua tese de doutorado intitulada \u201cVaria\u00e7\u00f5es dos metab\u00f3litos prim\u00e1rios de esp\u00e9cies de Rhodophyta das regi\u00f5es Ant\u00e1rtica e subant\u00e1rtica\u201d. A banca examinadora foi composta pela orientadora Prof. [&hellip;]<\/p>\n","protected":false},"author":187,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/8669"}],"collection":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/users\/187"}],"replies":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/comments?post=8669"}],"version-history":[{"count":3,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/8669\/revisions"}],"predecessor-version":[{"id":8674,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/8669\/revisions\/8674"}],"wp:attachment":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/media?parent=8669"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}