{"id":8627,"date":"2023-04-11T09:06:27","date_gmt":"2023-04-11T12:06:27","guid":{"rendered":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/?page_id=8627"},"modified":"2023-04-11T09:06:27","modified_gmt":"2023-04-11T12:06:27","slug":"ingrid-cristina-araujo-catarino-ms","status":"publish","type":"page","link":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/ingrid-cristina-araujo-catarino-ms\/","title":{"rendered":"Ingrid Cristina Araujo Catarino – MS"},"content":{"rendered":"

\"faixapos6\"<\/a><\/h2>\n
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Ingrid Cristina Araujo Catarino<\/h2>\n
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No dia 30 de Maio de 2020, a aluna da P\u00f3s-gradua\u00e7\u00e3o em Biodiversidade Vegetal e Meio Ambiente do Instituto de Bot\u00e2nica de S\u00e3o Paulo (IBt), Ingrid Cristina Araujo Catarino, defendeu sua disserta\u00e7\u00e3o de Mestrado intitulada: \u201cInflu\u00eancia da alta concentra\u00e7\u00e3o atmosf\u00e9rica de di\u00f3xido de carbono (\u2191[CO2]) e do d\u00e9ficit h\u00eddrico no ac\u00famulo de metab\u00f3litos secund\u00e1rios em folhas de Coffea arabica L.\u201d A banca examinadora foi composta pelo orientador Dr. Emerson Alves da Silva do N\u00facleo de Fisiologia e Bioqu\u00edmica (IBt), pelo Dr. Douglas Silva Domingues do Instituto de Bioci\u00eancias (UNESP \u2013 Rio Claro) e pelo Dr. Danilo C. Centeno do Centro de Ci\u00eancias Naturais e Humanas (UFABC \u2013 S\u00e3o Bernardo do Campo).<\/p>\n

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Influ\u00eancia da alta concentra\u00e7\u00e3o atmosf\u00e9rica de di\u00f3xido de carbono (\u2191[CO2]) e do d\u00e9ficit h\u00eddrico no ac\u00famulo de metab\u00f3litos secund\u00e1rios em folhas de Coffea arabica L<\/em>.<\/strong><\/h3>\n
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ABSTRACT<\/strong><\/p>\n

The increase in the atmospheric carbon dioxide concentration (\u2191[CO2<\/sub>]atm<\/sub>) is not an isolated effect, but has been accompanied by other changes in the climate, among them, increases in air temperature, which in turn contribute to the changes in precipitation patterns, causing more frequent and intense drought events. In coffee producing regions, drought is considered the main abiotic stress on coffee development and production, modifying its metabolism and final production. The coffee tree has a broad phytochemical composition, highlighting 5- caffeoylquinic acid and caffeine which, in addition to acting in the defence of plants, are important secondary compounds in the final cup quality. This work aimed to evaluate the effect of \u2191[CO2<\/sub>]atm<\/sub> and water deficit in gas exchange and accumulation of secondary metabolites in coffee trees (Coffea arabica<\/em> L.), in an early stage of growth. One hundred and twenty coffee plants were grown in Open Top Chambers (OTCs) facilities, under the concentrations of CO2<\/sub> of 400 ppm – CO2amb<\/sub> and 800 ppm – \u2191CO2<\/sub> and submitted to water regimes of daily watered (RD) and total water withholding (DH) for 40 days, constituting the following treatments: CO2amb<\/sub>RD, CO2amb<\/sub>DH, \u2191CO2<\/sub>RD and \u2191CO2<\/sub>DH. Measurements of leaf water potential (\u03a8wf<\/sub>), net carbon assimilation rates (A<\/em>, \u03bcmol CO2<\/sub> m-2 <\/sup>s-1<\/sup>), stomatal conductance (gs<\/em>, \u03bcmol m-2 <\/sup>s-1<\/sup>), transpiration (E<\/em>, mmol m-2<\/sup> s-1<\/sup>) and biochemical analyses of leaf phenolic compounds (CF) and total free amino acids (AA), as well as 5-caffeoylquinic acid (5-CQA) and caffeine by highperformance liquid chromatography, were carried out. The results showed that the water d\u00e9ficit imposed by the water withholding was sufficient to reduce the soil moisture in ca. 85% compared to treatments daily watered. The \u03a8wf<\/sub>, in turn, showed reductions of ca. 42% and 56% in the CO2amb<\/sub>DH and \u2191CO2<\/sub>DH treatments respectively. The performance of gas exchanges, especially the higher values of A were observed in coffee plants grown under high CO2<\/sub>, even under water restriction, suggesting that \u2191[CO2]atm could buffer the negative effects of DH. The increase in AA concentration in DH treatments indicates an osmoregulatory response to the imposed stress. The variations in the levels of CF, 5-CQA and caffeine, point to an increase in these substances in plants under DH, but without significant differences between treatments throughout the experiment, suggesting that in the initial growth stage of coffee plants, \u2191[CO2<\/sub>]atm<\/sub> does not affect the leaf accumulation of these substances, in the tested climate change scenario.<\/p>\n

Keywords:<\/strong> Climate change; 5-caffeoylquinic acid; Caffeine; Photosynthesis.<\/p>\n

RESUMO<\/strong><\/p>\n

O aumento da concentra\u00e7\u00e3o de di\u00f3xido de carbono atmosf\u00e9rico (\u2191[CO2<\/sub>]atm<\/sub>) n\u00e3o constitui um efeito isolado, mas vem acompanhado por outras altera\u00e7\u00f5es no clima, dentre elas, os aumentos na temperatura do ar, que por seu turno contribuem para as altera\u00e7\u00f5es nos padr\u00f5es de precipita\u00e7\u00e3o, podendo ocasionar secas mais frequentes e intensas. Nas regi\u00f5es produtoras de caf\u00e9, a seca \u00e9 considerada o principal estresse abi\u00f3tico capaz de afetar o desenvolvimento e produ\u00e7\u00e3o do cafeeiro, pois modifica o seu metabolismo e, consequentemente, sua produ\u00e7\u00e3o. O cafeeiro possui uma composi\u00e7\u00e3o fitoqu\u00edmica ampla, destacando o \u00e1cido 5-cafeiolqu\u00ednico e a cafe\u00edna que, al\u00e9m de atuarem na defesa das plantas, s\u00e3o compostos secund\u00e1rios importantes na qualidade final da bebida. O objetivo desse trabalho foi avaliar o efeito conjunto da \u2191[CO2<\/sub>]atm<\/sub> e d\u00e9ficit h\u00eddrico nas trocas gasosas e ac\u00famulo de metab\u00f3litos secund\u00e1rios em cafeeiros (Coffea<\/em> arabica <\/em>L.), em est\u00e1gio inicial de crescimento. Cento e vinte cafeeiros foram cultivados em C\u00e2maras de Topo Aberto (OTCs), sob as concentra\u00e7\u00f5es de CO2<\/sub> de 400 ppm – CO2<\/sub>amb e 800 ppm – \u2191CO2<\/sub>, e submetidos aos regimes h\u00eddricos de regas di\u00e1rias (RD) e suspens\u00e3o total de rega (DH) no per\u00edodo de 40 dias, constituindo os seguintes tratamentos: [CO2<\/sub>]amb<\/sub>RD, [CO2<\/sub>]amb<\/sub>DH, \u2191[CO2<\/sub>]RD e \u2191[CO2<\/sub>]DH. Foram realizadas medidas do potencial da \u00e1gua nas folhas (\u03a8wf) da taxa de assimila\u00e7\u00e3o l\u00edquida do carbono (A<\/em>, \u03bcmol CO2<\/sub> m-2<\/sup> s-1<\/sup>), condut\u00e2ncia estom\u00e1tica (g<\/em>s<\/sub>, \u03bcmol m-2<\/sup> s-1<\/sup>), transpira\u00e7\u00e3o (E<\/em>, mmol m-2<\/sup> s-1<\/sup>),) e an\u00e1lises bioqu\u00edmicas foliares para quantifica\u00e7\u00e3o de compostos fen\u00f3licos (CF) e amino\u00e1cidos livres totais (AA), bem como, \u00e1cido 5-cafeoilqu\u00ednico (5-CQA) e cafe\u00edna por cromatografia l\u00edquida de alta efici\u00eancia. Os resultados demonstraram que o d\u00e9ficit h\u00eddrico imposto pela suspens\u00e3o de rega foi suficiente para reduzir a umidade do solo em ca. de 85% em rela\u00e7\u00e3o aos tratamentos regados diariamente. O \u03a8wf<\/sub> por seu turno apresentou redu\u00e7\u00f5es de ca. de 42% e 56% nos tratamentos CO2amb<\/sub>DH e \u2191CO2<\/sub>DH respectivamente. A performance das trocas gasosas, em especial os maiores valores de A <\/em>foram observados nos cafeeiros cultivados sob alto CO2, mesmo sob restri\u00e7\u00e3o h\u00eddrica, sugerindo que a \u2191[CO2<\/sub>]atm<\/sub> pode tamponar os efeitos negativos do DH. O aumento das concentra\u00e7\u00f5es de AA nos tratamentos sob DH indicam esses compostos atuaram como osmorreguladores ao estresse imposto. As varia\u00e7\u00f5es nos n\u00edveis de CF, 5-CQA e cafe\u00edna, apontam para um aumento dessas subst\u00e2ncias nas plantas sob DH, por\u00e9m sem diferen\u00e7as significativas entre os tratamentos ao longo do experimento, sugerindo que no est\u00e1gio inicial de crescimento das plantas de caf\u00e9, o \u2191[CO2<\/sub>]atm<\/sub> n\u00e3o afeta o ac\u00famulo dessas subst\u00e2ncias nas folhas, no cen\u00e1rio de mudan\u00e7as clim\u00e1ticas testado.<\/p>\n

Palavras-chave:<\/strong> Mudan\u00e7as Clim\u00e1ticas; \u00e1cido 5-caafeoilquinico; Cafe\u00edna; fotoss\u00edntese.<\/p>\n

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\"pdf_grande\"<\/a>Ingrid Cristina Araujo Catarino<\/strong><\/a>
\nInflu\u00eancia da alta concentra\u00e7\u00e3o atmosf\u00e9rica de di\u00f3xido de carbono (\u2191[CO2]) e do d\u00e9ficit h\u00eddrico no ac\u00famulo de metab\u00f3litos secund\u00e1rios em folhas de Coffea arabica L<\/em>.<\/a><\/p>\n

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\u00a0VOLTAR AS DISSERTA\u00c7\u00d5ES E TESES<\/a><\/strong><\/p>\n

\n","protected":false},"excerpt":{"rendered":"

Ingrid Cristina Araujo Catarino No dia 30 de Maio de 2020, a aluna da P\u00f3s-gradua\u00e7\u00e3o em Biodiversidade Vegetal e Meio Ambiente do Instituto de Bot\u00e2nica de S\u00e3o Paulo (IBt), Ingrid Cristina Araujo Catarino, defendeu sua disserta\u00e7\u00e3o de Mestrado intitulada: \u201cInflu\u00eancia da alta concentra\u00e7\u00e3o atmosf\u00e9rica de di\u00f3xido de carbono (\u2191[CO2]) e do d\u00e9ficit h\u00eddrico no ac\u00famulo […]<\/p>\n","protected":false},"author":187,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/8627"}],"collection":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/users\/187"}],"replies":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/comments?post=8627"}],"version-history":[{"count":1,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/8627\/revisions"}],"predecessor-version":[{"id":8628,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/8627\/revisions\/8628"}],"wp:attachment":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/media?parent=8627"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}