{"id":8569,"date":"2023-04-06T12:54:31","date_gmt":"2023-04-06T15:54:31","guid":{"rendered":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/?page_id=8569"},"modified":"2023-04-06T12:55:34","modified_gmt":"2023-04-06T15:55:34","slug":"cynthia-murakami-dr","status":"publish","type":"page","link":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/cynthia-murakami-dr\/","title":{"rendered":"Cynthia Murakami – DR"},"content":{"rendered":"

\"\"<\/a><\/p>\n

\n

Cynthia Murakami<\/h2>\n
\n

No dia 26 de outubro de 2017, a aluna Cynthia Murakami defendeu a sua tese de doutorado no Instituto de Bot\u00e2nica intitulada \u201cDiversidade qu\u00edmica e potencial farmacol\u00f3gico de Hedyosmum brasiliense<\/em> Mart. ex Miq.\u201d.<\/p>\n

\"\"A banca examinadora foi composta pela sua orientadora Dra. Maria Cl\u00e1udia Marx Young e pelos pesquisadores Dra. Ivana Barbosa Suffredini (UNIP), Dra. Patr\u00edcia Sartorelli (UNIFESP), Dra. Marcia Ortiz Mayo Marques (IAC) e Dra. Deborah Yara Alves Cursino dos Santos (IB \/ USP).<\/p>\n

\n

Diversidade qu\u00edmica e potencial farmacol\u00f3gico de Hedyosmum brasiliense<\/em> Mart. ex Miq.<\/strong><\/h3>\n
\n

ABSTRACT<\/strong><\/h4>\n

Hedyosmum brasiliense<\/em> (Chloranthaceae), popularly known as \u201cch\u00e1-de-bugre\u201d, is a dioecious shrub. Its leaves has been used in folk medicine to treat of migraine, ovarium dysfunction, foot fungi, rheumatism, stomach pain and as diuretic. Studies have demonstrated aphrodisiac, analgesic, anxiolytic and antidepressive properties of their sesquiterpene lactones, as well as the antimicrobial and antifungal potential of their essential oils. The study of medicinal plants under different aspects has evidenced that their chemical composition may vary according to the geographic location, phenological phase and even gender in dioecious plants, which could interfere in their pharmacological potential. Since several factors could influence in the chemical composition of plants, the essential oils of fresh and dried leaves, flowers and fruits of male and female plants of H<\/em>.<\/em> brasiliense<\/em> collected in different phenological phases and from Atlantic Forest and Cerrado were extracted and tested for antifungal, antimicrobial, antitumor, antioxidant and anticholinesterase assays. PCA allowed discriminating the essential oils of plants from Atlantic Forest and Cerrado, as well as from flowering phase, but not from male and female leaves. The essential oils from Atlantic Forest were more active against antimicrobial, antifungal and cytotoxic activities when compared to those from Cerrado. No significant phenological variation was observed in the cytotoxic activity of the fresh leaves essential oils from Atlantic Forest. The essential oils tested were inactive for the anticholinesterase assay and antioxidant by the DPPH radical scavenging method but showed weak b-carotene\/linoleic acid antioxidant activity. Fixed metabolites of male and female plants of H<\/em>.<\/em> brasiliense<\/em> from Atlantic Forest and Cerrado were compared by 1<\/sup>H-NMR and PCA, CLAED-DAD and LC-MS\/MS and tested for antifungal, antibacterial and cytotoxic activities. Crude extracts of male and female plants were similar in both populations, but crude extracts from Atlantic Forest were selected for fractionation and purification as they presented stronger antifungal activity when compared to those from Cerrado. Eight pure compounds were isolated and identified, of which two are unpublished, the sesterpene hedyosulide and the esther pinocarvyl vanillate. The sesquiterpene lactones hedyosumin D, 15-acetoxy-isogermafurenolide, linderolide F, neolitacumone B and oxyonoseriolide, and the bisnor-sesquiterpene blumenol B were also isolated. Three identified components presented antifungal activity, hedyosumin D, linderolide F and neolitacumone B. The crude extracts of both populations presented cytotoxic potential, which remained in the hexane and dichloromethane extracts of the Atlantic Forest population. The crude extracts of both populations and the hexane extract of the Atlantic Forest population showed antibacterial potential against gram-positive bacteria.<\/p>\n

Keywords<\/strong>: biological activities, phenological phases, Cerrado, Atlantic Forest, \u201cParque Natural Municipal Nascentes de Paranapiacaba\u201d, essential oils, sesquiterpene lactones, sesterpene.<\/p>\n

RESUMO<\/strong><\/h4>\n

Hedyosmum brasiliense<\/em> (Chloranthaceae), popularmente conhecida como ch\u00e1-de-bugre, \u00e9 um arbusto dioico cujas folhas s\u00e3o utilizadas na medicina popular para o tratamento de enxaqueca, disfun\u00e7\u00f5es do ov\u00e1rio, frieira, reumatismo, dores de est\u00f4mago e como diur\u00e9tico. Estudos comprovaram as propriedades afrodis\u00edaca, analg\u00e9sica, ansiol\u00edtica e antidepressiva de suas lactonas sesquiterp\u00eanicas, bem como o potencial antimicrobiano e antif\u00fangico de seus \u00f3leos essenciais. O estudo de plantas medicinais sob diferentes aspectos tem evidenciado que a sua composi\u00e7\u00e3o qu\u00edmica pode variar de acordo com a localiza\u00e7\u00e3o geogr\u00e1fica, a fase fenol\u00f3gica e at\u00e9 o g\u00eanero em plantas dioicas, podendo interferir no seu potencial farmacol\u00f3gico. Uma vez que a composi\u00e7\u00e3o qu\u00edmica pode variar por diversos fatores, os \u00f3leos essenciais de folhas frescas e secas, flores e frutos de plantas masculinas e femininas de H<\/em>.<\/em> brasiliense<\/em> coletadas em diferentes fases fenol\u00f3gicas e provenientes de Paranapiacaba (Mata Atl\u00e2ntica) e Mogi Gua\u00e7u (Cerrado) foram extra\u00eddos e testados para os ensaios antif\u00fangico, antimicrobiano, antitumoral, antioxidante e anticolinester\u00e1sico. A ACP permitiu discriminar os \u00f3leos essenciais das plantas provenientes da Mata Atl\u00e2ntica e do Cerrado, bem como da fase de flora\u00e7\u00e3o, mas n\u00e3o distinguiu folhas masculinas e femininas. Os \u00f3leos essenciais provenientes da Mata Atl\u00e2ntica foram mais ativos frente \u00e0s atividades antimicrobiana, antif\u00fangica e citot\u00f3xica, quando comparados com os \u00f3leos essenciais das plantas provenientes do Cerrado. N\u00e3o foi observada varia\u00e7\u00e3o fenol\u00f3gica significativa na atividade citot\u00f3xica dos \u00f3leos essenciais de folhas frescas da Mata Atl\u00e2ntica. Os \u00f3leos essenciais testados foram inativos para a atividade anticolinester\u00e1sica e antioxidante pelo m\u00e9todo de sequestro do DPPH mas apresentaram atividade fraca pelo m\u00e9todo do b-caroteno\/\u00e1cido linol\u00e9ico. Os componentes fixos de plantas masculinas e femininas de H<\/em>.<\/em> brasiliense<\/em> coletadas em Mata Atl\u00e2ntica e Cerrado tamb\u00e9m foram comparados pelos m\u00e9todos de 1<\/sup>H-RMN e ACP, CLAED-DAD e LC-MS\/MS e testados para as atividades antif\u00fangica, antibacteriana e citot\u00f3xica. Os extratos brutos de plantas masculinas e femininas foram semelhantes em ambas as popula\u00e7\u00f5es, por\u00e9m os extratos da Mata Atl\u00e2ntica foram selecionados para fracionamento e purifica\u00e7\u00e3o por apresentarem forte potencial antif\u00fangico quando comparado aos do Cerrado. Foram isoladas e identificadas oito subst\u00e2ncias puras, das quais duas s\u00e3o in\u00e9ditas na literatura, o sesterterpeno hedyosulida e o \u00e9ster vanilato de pinocarvila. Tamb\u00e9m foram isoladas as lactonas sesquiterp\u00eanicas hedyosumina D, 15-acetoxi-isogermafurenol\u00eddeo, linderol\u00eddeo F, neolitacumona B e oxionoseriol\u00eddeo, al\u00e9m do bisnor-sesquiterpeno blumenol B. Tr\u00eas componentes identificados apresentaram atividade antif\u00fangica, hedyosumina D, linderol\u00eddeo F e neolitacumona B. Os extratos brutos de ambas as popula\u00e7\u00f5es apresentaram potencial citot\u00f3xico, que se manteve nos res\u00edduos das parti\u00e7\u00f5es em hexano e diclorometano da popula\u00e7\u00e3o da Mata Atl\u00e2ntica. Os extratos brutos de ambas as popula\u00e7\u00f5es e res\u00edduos da parti\u00e7\u00e3o em hexano da popula\u00e7\u00e3o da Mata Atl\u00e2ntica apresentaram potencial antibacteriano contra bact\u00e9rias gram-positivas.<\/p>\n

Palavras-chave:<\/strong> atividades biol\u00f3gicas, fases fenol\u00f3gicas, Cerrado, Mata Atl\u00e2ntica, Parque Natural Municipal Nascentes de Paranapiacaba, \u00f3leos essenciais, lactonas sesquiterp\u00eanicas, sesterterpeno.<\/p>\n

\n

\"pdf_grande\"<\/a>Cynthia Murakami
\nDiversidade qu\u00edmica e potencial farmacol\u00f3gico de Hedyosmum brasiliense<\/em> Mart. ex Miq.<\/a><\/p>\n

\n

\u00a0VOLTAR AS DISSERTA\u00c7\u00d5ES E TESES<\/a><\/strong><\/p>\n

\n","protected":false},"excerpt":{"rendered":"

Cynthia Murakami No dia 26 de outubro de 2017, a aluna Cynthia Murakami defendeu a sua tese de doutorado no Instituto de Bot\u00e2nica intitulada \u201cDiversidade qu\u00edmica e potencial farmacol\u00f3gico de Hedyosmum brasiliense Mart. ex Miq.\u201d. A banca examinadora foi composta pela sua orientadora Dra. Maria Cl\u00e1udia Marx Young e pelos pesquisadores Dra. Ivana Barbosa Suffredini […]<\/p>\n","protected":false},"author":187,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/8569"}],"collection":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/users\/187"}],"replies":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/comments?post=8569"}],"version-history":[{"count":2,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/8569\/revisions"}],"predecessor-version":[{"id":8574,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/8569\/revisions\/8574"}],"wp:attachment":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/media?parent=8569"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}