{"id":6964,"date":"2019-06-24T11:28:59","date_gmt":"2019-06-24T14:28:59","guid":{"rendered":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/?page_id=6964"},"modified":"2019-06-24T11:30:45","modified_gmt":"2019-06-24T14:30:45","slug":"sabrina-vanessa-de-andrade-dos-santos-dr","status":"publish","type":"page","link":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/dissertacoesteses\/sabrina-vanessa-de-andrade-dos-santos-dr\/","title":{"rendered":"Sabrina Vanessa de Andrade dos Santos DR"},"content":{"rendered":"

\"faixapos6\"<\/a><\/h2>\n
\n

Sabrina Vanessa de Andrade dos Santos<\/h2>\n
\n

No dia 27 de novembro de 2018, Sabrina Vanessa de Andrade dos Santos, aluna de p\u00f3s-gradua\u00e7\u00e3o em Biodiversidade Vegetal e Meio Ambiente do Instituto de Bot\u00e2nica da Secretaria do Meio Ambiente do Estado de S\u00e3o Paulo, bolsista CNPq, sob orienta\u00e7\u00e3o da Dra. V\u00edvian Tamaki e co-orienta\u00e7\u00e3o da Dra. Silvia Ribeiro de Souza, defendeu sua tese de Doutorado intitulada:\u00a0\u201cNitrog\u00eanio, alta radia\u00e7\u00e3o fotossinteticamente ativa e UV-B na indu\u00e7\u00e3o da colora\u00e7\u00e3o rubra na brom\u00e9lia Alcantarea imperialis <\/em>(Carri\u00e8re) Harms\u201d.<\/p>\n

A banca examinadora foi composta pela Dra. Regina Maria de Moraes (N\u00facleo de Pesquisa em Ecologia \u2013 IBt), Dr. Marcos Pereira Marinho Aidar (N\u00facleo de Pesquisa em Fisiologia e Bioqu\u00edmica de Plantas \u2013 IBt), Dr. Shoey Kanashiro (N\u00facleo de Pesquisa em Plantas Ornamentais \u2013 IBt), Dra. Daniela Soares dos Santos (Centro Paula Souza) e Dra. V\u00edvian Tamaki (Orientadora, N\u00facleo de Pesquisa em Plantas Ornamentais \u2013 IBt).<\/p>\n

\n

Nitrog\u00eanio, alta radia\u00e7\u00e3o fotossinteticamente ativa e UV-B na indu\u00e7\u00e3o da colora\u00e7\u00e3o rubra na brom\u00e9lia Alcantarea imperialis <\/em>(Carri\u00e8re) Harms<\/h3>\n
\n

ABSTRACT<\/strong><\/h4>\n

Alcantarea imperialis<\/em> (Carri\u00e8re) Harms is a large bromeliad species with showy inflorescence widely used in landscape projects. When grown in vitro<\/em> under different nitrogen (N) concentrations, its normally green leaves show red coloration, as well as some individuals in a natural environment. This red coloration, in addition to making it more ornamental, indicates the presence of anthocyanins, pigments that can be synthesized in response to environmental stimuli such as light, nutrient availability, temperature and UV radiation. Thus, the aim of this study to induce the accumulation of anthocyanins in A. imperialis<\/em> plants cultivated in vitro<\/em>, in different sources and concentrations of nitrogen (N), in high photosynthetically active radiation (PAR) or with ultraviolet radiation (UV-B), aiming to maintain the coloration, with satisfactory growth and development, during the acclimatization to produce specimens that have greater economic interest. Plants obtained from seeds were cultivated in vitro<\/em> and transferred to three experiments – in vitro<\/em> culture in Murashige & Skoog (MS) medium with varying concentrations (3.75, 7.5 and 15 mM) and nitrogen sources (NO3<\/sub>–<\/sup>, NH4<\/sub>+<\/sup> and NH4<\/sub>NO3<\/sub>) and control treatment (TC \u2013 60 mM N) – in vitro<\/em> culture in TC under high PAR (700 \u03bcmol.m-2<\/sup>.s-1<\/sup>) – culture in different intensities UV-B chamber (7.4 11.8 and 101.9 kJ.m-2<\/sup>.h-1<\/sup>) under fertilization with the same TC formulation. After six months the parameters number of leaves and roots, length of aerial and radicular parts, mass of aerial and root parts, photosynthetic pigment contents (chlorophyll a<\/em>, chlorophyll b<\/em> and carotenoids), anthocyanins and superoxide dismutase enzyme (SOD), catalase (CAT), glutathione reductase (GR) and ascorbate peroxidase (APX) were determined. Plants that accumulated anthocyanins were transferred to greenhouse, where they remained for 12 months to follow the color during acclimatization. Plants grown in vitro<\/em> at 3.75 and 7.5 mM of NO3<\/sub>–<\/sup> and NH4<\/sub>NO3<\/sub> were the most accumulated anthocyanins. The high PAR did not significantly influence the pigment accumulation, only 10% of the exposed plants showed anthocyanins in leaves. The different UV-B intensities also influenced the pigment accumulation in the treatments 4.7 and 101.9 kJ.m-2<\/sup>.h-1<\/sup>. High performance liquid chromatography (HPLC) identified pelargonidin, cyanidin and delphinidin glycosides on A. imperialis<\/em> leaves. The plants that were acclimatized maintained color during the 12 months, and the highest levels of anthocyanins resulted from the plants cultivated under fertilization with NH4<\/sub>NO3<\/sub>. The results indicated that the most suitable cultivation to obtain A. imperialis<\/em> red plants is the in vitro<\/em> in MS medium with the concentration of 15 mM NH4<\/sub>NO3<\/sub>, because in this concentration larger plants were obtained during the in vitro<\/em> phase and greater accumulation of anthocyanins after acclimatization.
\nKeywords<\/strong>: Bromeliaceae, anthocyanins, greenhouse, cyanidin, in vitro<\/em> culture, nitrogen sources<\/p>\n

 <\/p>\n

RESUMO<\/strong><\/h4>\n

Alcantarea imperialis<\/em> (Carri\u00e8re) Harms \u00e9 uma brom\u00e9lia de grande porte com infloresc\u00eancia vistosa muito utilizada em projetos paisag\u00edsticos. Quando cultivada in vitro<\/em> sob diferentes concentra\u00e7\u00f5es de nitrog\u00eanio (N), suas folhas normalmente verdes apresentam colora\u00e7\u00e3o vermelha, assim como alguns indiv\u00edduos em ambiente natural. Essa colora\u00e7\u00e3o rubra, al\u00e9m de torn\u00e1-la mais ornamental, indica a presen\u00e7a de antocianinas, pigmentos que podem ser sintetizados em resposta a est\u00edmulos ambientais, como luz, disponibilidade de nutrientes, temperatura e radia\u00e7\u00e3o UV. Sendo assim, o objetivo deste trabalho foi induzir o ac\u00famulo de antocianinas em plantas de A. imperialis<\/em> cultivadas in vitro, <\/em>em diferentes fontes e concentra\u00e7\u00f5es de nitrog\u00eanio (N), na presen\u00e7a de alta radia\u00e7\u00e3o fotossinteticamente ativa (PAR) ou com radia\u00e7\u00e3o ultravioleta (UV-B), visando manter a colora\u00e7\u00e3o, com crescimento e desenvolvimentos satisfat\u00f3rios, durante a aclimatiza\u00e7\u00e3o para a produ\u00e7\u00e3o de exemplares que possuam maior interesse econ\u00f4mico. Plantas obtidas a partir de sementes foram transferidas para tr\u00eas experimentos \u2013 cultivo in vitro<\/em> em meio de Murashige & Skoog (MS) com modifica\u00e7\u00f5es nas concentra\u00e7\u00f5es (3,75, 7,5 e 15 mM) e fontes nitrogenadas \u200b\u200b(NO3<\/sub>–<\/sup>, NH4<\/sub>+<\/sup> e NH4<\/sub>NO3<\/sub>) e tratamento controle (TC \u2013 60 mM de N) \u2013 cultivo in vitro<\/em> em TC sob alta PAR (700 \u00b5mol.m-2<\/sup>.s-1<\/sup>) \u2013 cultivo em c\u00e2maras com diferentes intensidade de UV-B (4,7 11,8 e 101,9 kJ.m-2<\/sup>.h-1<\/sup>) sob aduba\u00e7\u00e3o com a mesma formula\u00e7\u00e3o do TC. Ap\u00f3s 6 meses foram avaliados os par\u00e2metros n\u00famero de folhas e ra\u00edzes, comprimento foliar e radicular, massa das partes a\u00e9rea e radicular, teores de pigmentos fotossint\u00e9ticos (clorofila a<\/em>, b<\/em> e carotenoides), antocianinas e as atividades das enzimas super\u00f3xido dismutase (SOD), catalase (CAT), glutationa redutase (GR) e ascorbato peroxidase (APX). Plantas que acumularam antocianinas foram transferidas para casa de vegeta\u00e7\u00e3o, onde permaneceram por 12 meses para acompanhamento da cor durante a aclimatiza\u00e7\u00e3o. Constatou-se que as plantas cultivadas in vitro<\/em> nas concentra\u00e7\u00f5es 3,75 e 7,5 mM de NO3<\/sub> e NH4<\/sub>NO3 <\/sub>foram as que mais acumularam antocianinas. A alta PAR n\u00e3o influenciou de forma significativa no ac\u00famulo do pigmento, apenas 10% das plantas expostas apresentaram antocianinas foliares. As diferentes intensidades de UV-B tamb\u00e9m influenciaram no ac\u00famulo do pigmento nos tratamentos\u00a0 4,7 e 101,9 kJ.m-2<\/sup>.h-1<\/sup>. Foram identificados por cromatografia l\u00edquida de alta efici\u00eancia (HPLC) os glicos\u00eddeos de pelargonidina, cianidina e delfinidina em folhas de A. imperialis<\/em>. As plantas que foram aclimatizadas mantiveram a colora\u00e7\u00e3o durante os 12 meses de acompanhamento, sendo que os maiores teores de antocianinas resultaram das plantas cultivadas sob aduba\u00e7\u00e3o com NH4<\/sub>NO3<\/sub>. Os resultados indicaram que o cultivo mais adequado para obten\u00e7\u00e3o de plantas rubras de A. imperialis <\/em>\u00e9 o in vitro <\/em>em meio MS com a concentra\u00e7\u00e3o de 15 mM de NH4<\/sub>NO3, <\/sub>pois nesta concentra\u00e7\u00e3o obteve-se plantas maiores durante a fase in vitro <\/em>e maior ac\u00famulo de antocianinas ap\u00f3s a aclimatiza\u00e7\u00e3o.
\nPalavras-chave<\/strong>: Bromeliaceae, antocianinas, casa de vegeta\u00e7\u00e3o, cianidina, cultivo in vitro<\/em>,\u00a0 fontes de nitrog\u00eanio<\/p>\n

<\/h4>\n
\n

\"pdf_grande\"<\/a>Sabrina Vanessa de Andrade dos Santos<\/span><\/a>
\nNitrog\u00eanio, alta radia\u00e7\u00e3o fotossinteticamente ativa e UV-B na indu\u00e7\u00e3o da colora\u00e7\u00e3o rubra na brom\u00e9lia Alcantarea imperialis <\/em>(Carri\u00e8re) Harms<\/span><\/a><\/p>\n

\n

\u00a0VOLTAR AS DISSERTA\u00c7\u00d5ES E TESES<\/a><\/strong><\/p>\n

\n","protected":false},"excerpt":{"rendered":"

Sabrina Vanessa de Andrade dos Santos No dia 27 de novembro de 2018, Sabrina Vanessa de Andrade dos Santos, aluna de p\u00f3s-gradua\u00e7\u00e3o em Biodiversidade Vegetal e Meio Ambiente do Instituto de Bot\u00e2nica da Secretaria do Meio Ambiente do Estado de S\u00e3o Paulo, bolsista CNPq, sob orienta\u00e7\u00e3o da Dra. V\u00edvian Tamaki e co-orienta\u00e7\u00e3o da Dra. Silvia […]<\/p>\n","protected":false},"author":103,"featured_media":0,"parent":249,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/6964"}],"collection":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/users\/103"}],"replies":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/comments?post=6964"}],"version-history":[{"count":2,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/6964\/revisions"}],"predecessor-version":[{"id":6968,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/6964\/revisions\/6968"}],"up":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/249"}],"wp:attachment":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/media?parent=6964"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}