{"id":6732,"date":"2022-09-02T10:00:48","date_gmt":"2022-09-02T13:00:48","guid":{"rendered":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/?page_id=6732"},"modified":"2022-09-09T12:12:14","modified_gmt":"2022-09-09T15:12:14","slug":"athos-poli-riqui-dr","status":"publish","type":"page","link":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/athos-poli-riqui-dr\/","title":{"rendered":"Athos Poli Rigui &#8211; DR"},"content":{"rendered":"<h2 style=\"text-align: center\"><a href=\"arquivos.ambiente.sp.gov.br\/pgibt\/2015\/02\/faixapos6.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-3777 size-full\" style=\"border: 0px\" src=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/2015\/02\/faixapos6.jpg\" alt=\"faixapos6\" width=\"950\" height=\"163\" srcset=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/2015\/02\/faixapos6.jpg 950w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/2015\/02\/faixapos6-768x131.jpg 768w\" sizes=\"(max-width: 950px) 100vw, 950px\" \/><\/a><\/h2>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<h2 style=\"text-align: center\">Athos Poli Rigui<\/h2>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<p style=\"text-align: justify\">O aluno Athos Poli Rigui da Silva, bolsista CAPES e PDSE\/CAPES, obteve o t\u00edtulo de Doutor pelo Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biodiversidade Vegetal e Meio Ambiente do Instituto de Bot\u00e2nica de S\u00e3o Paulo, em 20 de fevereiro de 2019.\u00a0 Sua tese de doutorado intitulada: \u201cSinaliza\u00e7\u00e3o do \u00f3xido n\u00edtrico no metabolismo de frutanos e nas respostas antioxidantes em<em> Lolium perenne L<\/em>.\u201d teve orienta\u00e7\u00e3o da Dra. Mar\u00edlia Gaspar e co-orienta\u00e7\u00e3o da Dra. Maria Angela Machado de Carvalho.<\/p>\n<p style=\"text-align: justify\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-8199 size-full\" src=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2022\/09\/athos_poli_rigui_dr.jpg\" alt=\"\" width=\"1000\" height=\"486\" srcset=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2022\/09\/athos_poli_rigui_dr.jpg 1000w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2022\/09\/athos_poli_rigui_dr-320x156.jpg 320w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2022\/09\/athos_poli_rigui_dr-768x373.jpg 768w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2022\/09\/athos_poli_rigui_dr-250x122.jpg 250w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2022\/09\/athos_poli_rigui_dr-550x267.jpg 550w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2022\/09\/athos_poli_rigui_dr-800x389.jpg 800w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2022\/09\/athos_poli_rigui_dr-370x180.jpg 370w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2022\/09\/athos_poli_rigui_dr-617x300.jpg 617w\" sizes=\"(max-width: 1000px) 100vw, 1000px\" \/><\/p>\n<p style=\"text-align: justify\">A banca examinadora foi composta pela Dra. Mar\u00edlia Gaspar (IBt), Dra. Moemy Gomes de Moraes (UFG), Dra. Ione Salgado (IBt), Dr. Andr\u00e9 Luis Wendt dos Santos (USP) e Dra. M\u00e1rcia Regina Braga (IBt).<\/p>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<h3 style=\"text-align: center\">Sinaliza\u00e7\u00e3o do \u00f3xido n\u00edtrico no metabolismo de frutanos e nas respostas antioxidantes em <em>Lolium perenne L.<\/em><\/h3>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<h4 style=\"text-align: center\"><strong>ABSTRACT<\/strong><\/h4>\n<p style=\"text-align: justify\">Nitric oxide (NO), a reactive nitrogen species, is a signaling molecule in plant development, as well as in responses to biotic and abiotic stresses. These conditions trigger an oxidative stress, which can be minimized by applying low concentrations of exogenous NO, that modulates the activity of antioxidant enzymes through S-nitrosylation. Fructans are reserve carbohydrates with an important role in cold and drought tolerance by converting high polymerization fructans into oligosaccharides that act as osmoregulators, membrane protectors and antioxidant compounds. The interaction among NO and fructan metabolism there is a lack of discussion considering the role of these carbohydrates in tolerance to different environmental stresses and the role of NO as modulator of the activity of stress related enzymes. This work aimed to evaluate the responses of <em>Lolium perenne<\/em>, a fructan accumulator, to treatment with an NO donor solution (S-nitrosoglutathione, GSNO) under physiological conditions and under water deficit, in order to identify the predominant antioxidant route used by the plant: fructan metabolism or the enzymatic antioxidant system. We evaluated growth parameters and the activities and expressions of antioxidant and fructan metabolism enzymes. Plant growth was not affected by any of the applied treatments. Fructan metabolism enzymes, as well as the related genes, seemed to be regulated by water deficit treatment as well as GSNO and the reduced glutathione (GSH), used as control treatment, although these adjustments may occur by other routes previously regulated by such treatments. In contrast, generally responses of increased activities and the expression of genes related to the antioxidant metabolism are consistent with that has been described for other grasses under water deficit, as well as the regulation of NO expression and activity. Therefore, in response to water deficit, young plants of <em>L. perenne<\/em> were tolerant to water stress, reducing the ROS content, due to the increase in synthesis and accumulation of fructan and GSH.<\/p>\n<p style=\"text-align: justify\"><strong>Key words:<\/strong> Carbohydrates metabolism, S-nitrosylation, stress signaling, water deficit.<\/p>\n<h4 style=\"text-align: center\"><strong>RESUMO<\/strong><\/h4>\n<p style=\"text-align: justify\">O \u00f3xido n\u00edtrico (NO), uma das esp\u00e9cies reativas de nitrog\u00eanio, \u00e9 uma mol\u00e9cula sinalizadora no desenvolvimento de plantas, bem como em respostas a estresses bi\u00f3ticos e abi\u00f3ticos. Essas condi\u00e7\u00f5es desencadeiam um estresse oxidativo, que pode ser minimizado pela aplica\u00e7\u00e3o de baixas concentra\u00e7\u00f5es de NO ex\u00f3geno, que modula a atividade de enzimas antioxidantes por meio da S-nitrosila\u00e7\u00e3o ou e a express\u00e3o de genes relacionados. Os frutanos s\u00e3o carboidratos de reserva com um papel importante na toler\u00e2ncia ao frio e \u00e0 seca, atrav\u00e9s da convers\u00e3o de frutanos de alto grau de polimeriza\u00e7\u00e3o em oligossacar\u00eddeos que atuam como osmorreguladores, protetores de membrana e compostos antioxidantes. A intera\u00e7\u00e3o entre o NO e o metabolismo de frutanos \u00e9 pouco discutida considerando o papel desses carboidratos na toler\u00e2ncia a diferentes estresses ambientais e o papel do NO como modulador da atividade de enzimas relacionadas \u00e0 condi\u00e7\u00f5es de estresse. Este trabalho teve por objetivo avaliar as respostas de plantas de <em>Lolium perenne<\/em>, esp\u00e9cie acumuladora de frutanos, tratadas com solu\u00e7\u00e3o doadora de NO (S-nitrosoglutationa, GSNO) em condi\u00e7\u00f5es fisiol\u00f3gicas e sob d\u00e9ficit h\u00eddrico, visando identificar qual a via preferencial de resposta ao estresse: o metabolismo de frutanos ou o sistema antioxidante enzim\u00e1tico. Foram analisados par\u00e2metros de crescimento, atividade e express\u00e3o das enzimas do metabolismo de frutanos e antioxidante. O crescimento n\u00e3o foi afetado por nenhum tratamento. As enzimas do metabolismo de frutanos, bem como os genes relacionados, parecem ser regulados em condi\u00e7\u00e3o de estresse h\u00eddrico e tamb\u00e9m pelo tratamento com GSNO e com a glutationa reduzida (GSH), utilizada como controle, embora estas regula\u00e7\u00f5es possam ocorrer por outras vias previamente reguladas por tais tratamentos. Em contrapartida, em geral, as respostas de aumento da atividade e express\u00e3o dos genes do metabolismo antioxidante s\u00e3o condizentes com o descrito para outras gram\u00edneas sob d\u00e9ficit h\u00eddrico, bem como a regula\u00e7\u00e3o da express\u00e3o e da atividade por NO. Portanto, plantas jovens de<em> L. perenne var AberAvon<\/em> se mostraram resistentes ao estresse h\u00eddrico diminuindo o teor de ROS gra\u00e7as ao aumento da s\u00edntese e ac\u00famulo de frutanos e GSH.<\/p>\n<p style=\"text-align: justify\"><strong>Palavras chave: <\/strong>D\u00e9ficit h\u00eddrico, metabolismo de carboidratos, S-nitrosila\u00e7\u00e3o, sinaliza\u00e7\u00e3o de estresse.<\/p>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<p style=\"text-align: center\"><a href=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2022\/09\/athos_poli_rigui_da_silva_dr.pdf\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter\" src=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/2013\/09\/pdf_grande.gif\" alt=\"pdf_grande\" width=\"60\" height=\"60\" \/><\/a>Athos Poli Rigui<br \/>\n<a href=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/sites\/242\/2022\/09\/athos_poli_rigui_da_silva_dr.pdf\">Sinaliza\u00e7\u00e3o do \u00f3xido n\u00edtrico no metabolismo de frutanos e nas respostas antioxidantes em<em> Lolium perenne L.<\/em><\/a><\/p>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<p style=\"text-align: center\"><strong>\u00a0<a href=\"www.infraestruturameioambiente.sp.gov.br\/pgibt\/dissertacoesteses\/\">VOLTAR AS DISSERTA\u00c7\u00d5ES E TESES<\/a><\/strong><\/p>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n","protected":false},"excerpt":{"rendered":"<p>Athos Poli Rigui O aluno Athos Poli Rigui da Silva, bolsista CAPES e PDSE\/CAPES, obteve o t\u00edtulo de Doutor pelo Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biodiversidade Vegetal e Meio Ambiente do Instituto de Bot\u00e2nica de S\u00e3o Paulo, em 20 de fevereiro de 2019.\u00a0 Sua tese de doutorado intitulada: \u201cSinaliza\u00e7\u00e3o do \u00f3xido n\u00edtrico no metabolismo de frutanos [&hellip;]<\/p>\n","protected":false},"author":95,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/6732"}],"collection":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/users\/95"}],"replies":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/comments?post=6732"}],"version-history":[{"count":4,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/6732\/revisions"}],"predecessor-version":[{"id":8241,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/6732\/revisions\/8241"}],"wp:attachment":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/media?parent=6732"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}