{"id":6474,"date":"2018-06-06T11:43:02","date_gmt":"2018-06-06T14:43:02","guid":{"rendered":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/?page_id=6474"},"modified":"2018-06-06T11:46:20","modified_gmt":"2018-06-06T14:46:20","slug":"eunice-reis-batista-dr","status":"publish","type":"page","link":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/dissertacoesteses\/eunice-reis-batista-dr\/","title":{"rendered":"Eunice Reis Batista DR"},"content":{"rendered":"<h2 style=\"text-align: center\"><a href=\"arquivos.ambiente.sp.gov.br\/pgibt\/2015\/02\/faixapos6.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-3777 size-full\" style=\"border: 0px\" src=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/2015\/02\/faixapos6.jpg\" alt=\"faixapos6\" width=\"950\" height=\"163\" srcset=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/2015\/02\/faixapos6.jpg 950w, https:\/\/smastr16.blob.core.windows.net\/pgibt\/2015\/02\/faixapos6-768x131.jpg 768w\" sizes=\"(max-width: 950px) 100vw, 950px\" \/><\/a><\/h2>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<h2 style=\"text-align: center\">Eunice Reis Batista<\/h2>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<p style=\"text-align: center\">No dia 25 de fevereiro de 2015, a aluna da P\u00f3s-gradua\u00e7\u00e3o em Biodiversidade Vegetal e Meio Ambiente do Instituto de Bot\u00e2nica de S\u00e3o Paulo (IBt), Eunice Reis Batista defendeu sua tese de doutorado intitulada\u00a0\u201cRespostas fisiol\u00f3gicas e metab\u00f3licas de duas cultivares de <em>Coffea arabica<\/em> L. (Rubiaceae) submetidas a atmosferas enriquecidas em CO<sub>2<\/sub> em C\u00e2maras de Topo Aberto e sistema FACE&#8221;.<\/p>\n<p style=\"text-align: center\">A banca examinadora foi composta pela orientadora Dra. M\u00e1rcia R. Braga, Dra. Mar\u00edlia Gaspar (IBt), Dr. Oliveiro Guerreiro Filho (IAC-SP), Dr. Marcos Buckeridge (IB-USP) e Dr. Danilo Centeno (UFABC-SP).<\/p>\n<p style=\"text-align: center\">O trabalho teve por objetivos avaliar o efeito de atmosferas enriquecidas de CO<sub>2<\/sub> em <em>Coffea arabica <\/em>comparando-se as cultivares Catua\u00ed vermelho IAC-144 e Obat\u00e3 vermelho IAC-1669, respectivamente suscet\u00edvel e resistente \u00e0 ferrugem alaranjada, doen\u00e7a causada pelo fungo <em>H. vastatrix <\/em>e que provoca grandes preju\u00edzos aos produtores. Foram avaliadas as taxas de assimila\u00e7\u00e3o l\u00edquida de carbono (<em>A<\/em>) e teores de compostos do metabolismo prim\u00e1rio e secund\u00e1rio. Os resultados obtidos a partir do estudo, indicaram que o incremento da concentra\u00e7\u00e3o atmosf\u00e9rica de CO<sub>2 <\/sub>tanto em casa de vegeta\u00e7\u00e3o como sob condi\u00e7\u00f5es de campo, tiveram efeito positivo nas taxas de fotoss\u00edntese. Os teores de carboidratos, fen\u00f3licos sol\u00faveis e lignina foram influenciados significativamente no cultivo em OTCs, o que n\u00e3o ocorreu, de forma geral, na estrutura experimental FACE. O aumento do CO<sub>2 <\/sub>atmosf\u00e9rico n\u00e3o influenciou a intera\u00e7\u00e3o do\u00a0 pat\u00f3geno <em>H. vastatrix<\/em> com os cafeeiros cultivados em OTCs.<\/p>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<h3 style=\"text-align: center\">Respostas fisiol\u00f3gicas e metab\u00f3licas de duas cultivares de <em>Coffea arabica<\/em> L. (Rubiaceae) submetidas a atmosferas enriquecidas em CO<sub>2<\/sub> em C\u00e2maras de Topo Aberto e sistema FACE<\/h3>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<h4 style=\"text-align: center\"><strong>ABSTRACT<\/strong><\/h4>\n<p style=\"text-align: justify\">Coffee is one of the main comodities of the Brazilian agribusiness but most cultivars of <em>Coffea arabica<\/em> are susceptible to the fungus <em>Hemileia vastatrix<\/em>, the causal agent of the coffee rust disease, with negative impacts on coffee prodution.\u00a0 The current concerns with the scenario of climate changes have stimulated studies on impacts of environmental changes on coffee cultivation and its interaction with the pathogen. This work aimed to evaluate the effect of CO<sub>2<\/sub>-enriched atmospheres on two cultivars of <em>Coffea arabica<\/em>, comparing the suscetible cultivar red Catua\u00ed 144 IAC with the resistant one IAC 1669 red Obat\u00e3.\u00a0 Three different experiments were carried out, two of them in Open Top Chambers (OTCs) under ambient (380ppm) and high CO<sub>2<\/sub> atmosphere (760 ppm), for 100 and 45 days, respectively, and the second one with <em>H. vastatrix<\/em> inoculation. The third experiment was performed under field conditions in the first free air CO<sub>2<\/sub> enrichment (FACE) system in Latin America, installed in the Embrapa Meio Ambiente, Jaguari\u00fana, SP, Brazil, in which coffee cultivars were subjected to current concentration (~ 380ppm) and CO<sub>2<\/sub>-enriched atmosphere (550 ppm) for 27 months. We evaluated the net carbon assimilation rates and the contents of primary and secondary metabolites. In the first experiment conducted in OTCs, an increase in carbon assimilation was observed under high CO<sub>2 <\/sub>conditions, mainly in the cultivar Catua\u00ed, but without significant effects on growth. Carbohydrate levels increased until the 46<sup>th<\/sup> day, with accumulation of glucose and fructose in the Catua\u00ed cultivar when compared with the Obat\u00e3, which stored predominantly starch. The photosynthetic acclimation occurred simultaneously to the accumulation of starch, following the reduction in the levels of nitrogen in both cultivars. The levels of <sup>15<\/sup>N and C varied according to the availability of atmospheric CO<sub>2<\/sub>, with significant effects on isotopic composition (D<sup>13<\/sup>C) and carbon isotope discrimination (D<sup>13<\/sup>C). In the second experiment, the net photosynthetic assimilation was measured after infection by the fungus <em>H. vastatrix<\/em>, being higher in both cultivars under elevated CO<sub>2<\/sub>. Increased levels of total soluble sugars (AST), starch, soluble total phenolic (FST) and lignin and reduction of glucose, fructose, sucrose, raffinose, stachyose and reducing sugars (AR) were observed in susceptible coffee (Catua\u00ed) not inoculated with the pathogen. Plant inoculation with the pathogen led to a reduction in the levels of AST, glucose, fructose and lignina and increasing in AST and starch with no changes in sucrose, raffinose and stachyose. No changes were observed in the severity of symptoms caused by <em>H. vastatrix<\/em> on the susceptible cultivar under high CO<sub>2<\/sub> when compared to ambient atmospheric concentration. The third experiment indicated that the atmospheric CO<sub>2<\/sub> concentration in the FACE system did not influence significantly the foliar concentrations of carbohydrates, total soluble phenolic and lignin, in both cultivars over the period of 27 months. On the other hand, the increase in the atmospheric CO<sub>2<\/sub> led to increased of photosynthesis rates of the coffee trees, mainly during warm and rainy seasons, contributing to the elevation of the intrinsic water use efficiency. Additionally, the increased carbon isotope discrimination indicated positive influence of increased atmospheric CO<sub>2<\/sub> concentration in carbon assimilation in both cultivars. In conclusion, the coffee cultivars responded to the elevated atmospheric CO<sub>2<\/sub> concentration increasing photosynthesis, but this increase did not result in resistance improvement to the coffee rust fungus when coffee was grown on OTCs.<br \/>\n<strong>Keywords<\/strong>: Rubiaceae \u2013 elevated [CO<sub>2<\/sub>] &#8211;\u00a0 climate changes<\/p>\n<h4 style=\"text-align: center\"><strong>RESUMO<\/strong><\/h4>\n<p style=\"text-align: justify\">O caf\u00e9 \u00e9 um dos principais produtos de exporta\u00e7\u00e3o do agroneg\u00f3cio brasileiro, no entanto, as principais variedades de <em>Coffea arabica <\/em>cultivadas s\u00e3o suscet\u00edveis ao fungo biotr\u00f3fico <em>Hemileia vastatrix,<\/em> causador da ferrugem alaranjada, doen\u00e7a que provoca significativas perdas na produ\u00e7\u00e3o. A atual preocupa\u00e7\u00e3o com o cen\u00e1rio de mudan\u00e7as clim\u00e1ticas tem motivado a realiza\u00e7\u00e3o de estudos sobre impactos das altera\u00e7\u00f5es ambientais previstas no cultivo cafeeiro e na intera\u00e7\u00e3o planta-pat\u00f3geno. Este trabalho teve por objetivos avaliar o efeito de atmosferas enriquecidas de CO<sub>2<\/sub> em <em>Coffea arabica <\/em>comparando-se as cultivares Catua\u00ed vermelho IAC-144 e Obat\u00e3 vermelho IAC-1669, respectivamente suscet\u00edvel e resistente \u00e0 ferrugem. Foram conduzidos tr\u00eas experimentos, sendo dois deles em C\u00e2maras de Topo Aberto (Open Top Chambers, OTCs) nas concentra\u00e7\u00f5es atmosf\u00e9ricas ambiente (380ppm) e o de elevado CO<sub>2<\/sub> (760 ppm), com dura\u00e7\u00e3o de 100 e 45 dias, respectivamente, e com\u00a0\u00a0 inocula\u00e7\u00e3o dos cafeeiros pelo fungo <em>Hemileia vastatrix<\/em> apenas no segundo experimento. O 3\u00ba experimento foi realizado sob condi\u00e7\u00f5es de campo no primeiro sistema de enriquecimento atmosf\u00e9rico de CO<sub>2<\/sub> ao ar livre (FACE) da Am\u00e9rica Latina instalado no campus experimental da Embrapa Meio Ambiente de Jaguari\u00fana, SP, no qual os cafeeiros das duas cultivares foram submetidos a concentra\u00e7\u00f5es atual (~380ppm) e de 550 ppm de CO<sub>2<\/sub> ao longo de 27 meses de cultivo. No primeiro experimento, realizado em OTCs, foi observado aumento nas taxas de assimila\u00e7\u00e3o de carbono sob condi\u00e7\u00f5es de elevado CO<sub>2, <\/sub>principalmente na cultivar Catua\u00ed, por\u00e9m sem efeitos significativos sobre o crescimento.\u00a0 Os teores de carboidratos aumentaram at\u00e9 os 46 dias, sendo que a cv. Catua\u00ed acumulou maiores teores de glucose e frutose enquanto a cv. Obat\u00e3 acumulou maiores teores de amido. A aclimata\u00e7\u00e3o fotossint\u00e9tica ocorreu simultaneamente ao ac\u00famulo de amido, sendo observada redu\u00e7\u00e3o dos teores de nitrog\u00eanio nas duas cultivares. Os teores de <sup>15<\/sup>N e C variaram de acordo com a disponibilidade de CO<sub>2<\/sub> atmosf\u00e9rico, com reflexos significativos na composi\u00e7\u00e3o isot\u00f3pica (d<sup>13<\/sup>C) e de discrimina\u00e7\u00e3o isot\u00f3pica do carbono (D<sup>13<\/sup>C). No segundo experimento, as medidas de taxas de fotoss\u00edntese realizadas ap\u00f3s o ciclo da infec\u00e7\u00e3o pelo fungo <em>H. vastatrix<\/em> foram maiores em todos os cafeeiros cultivados na concentra\u00e7\u00e3o elevada de CO<sub>2<\/sub>. Tamb\u00e9m foram verificados aumentos dos teores de a\u00e7\u00facares sol\u00faveis totais (AST), amido, fen\u00f3licos sol\u00faveis totais (FST) e lignina e diminui\u00e7\u00e3o dos teores de glucose, frutose, sacarose, rafinose, estaquiose e de a\u00e7\u00facares redutores (AR) em cafeeiros suscet\u00edveis (Catua\u00ed) n\u00e3o inoculados com o pat\u00f3geno. Quando foram inoculados com o pat\u00f3geno, esses cafeeiros, apresentaram redu\u00e7\u00e3o dos teores de AST, glucose, frutose e lignina e eleva\u00e7\u00e3o dos teores de AR e amido e n\u00e3o apresentaram varia\u00e7\u00f5es nos teores de sacarose, rafinose e estaquiose. N\u00e3o foi observada altera\u00e7\u00e3o na severidade dos sintomas causados por <em>Hemileia vastatrix<\/em> nos cafeeiros suscet\u00edveis crescidos em alto de CO<sub>2 <\/sub>em compara\u00e7\u00e3o com aqueles mantidos na concentra\u00e7\u00e3o atmosf\u00e9rica ambiente. O 3\u00ba experimento indicou que o aumento da concentra\u00e7\u00e3o atmosf\u00e9rica de CO<sub>2<\/sub> nos cafeeiros cultivados no sistema FACE n\u00e3o influenciou significativamente as concentra\u00e7\u00f5es foliares de carboidratos, de fen\u00f3licos sol\u00faveis totais e de lignina, nas duas cultivares ao longo do per\u00edodo de 27 meses. Por outro lado, propiciou o aumento das taxas de fotoss\u00edntese do cafeeiro, principalmente no per\u00edodo quente e \u00famido, contribuindo ainda para a eleva\u00e7\u00e3o da efici\u00eancia intr\u00ednseca do uso da \u00e1gua. Adicionalmente, o aumento da discrimina\u00e7\u00e3o isot\u00f3pica de carbono indicou influ\u00eancia positiva da maior concentra\u00e7\u00e3o atmosf\u00e9rica de CO<sub>2<\/sub> na assimila\u00e7\u00e3o de carbono em todos os cafeeiros.\u00a0 Em resumo, as cultivares de cafeeiro estudadas responderam ao incremento da concentra\u00e7\u00e3o atmosf\u00e9rica de CO<sub>2 <\/sub>com aumento na fotoss\u00edntese, por\u00e9m este incremento n\u00e3o resultou em melhora de resist\u00eancia ao fungo causador da ferrugem do caf\u00e9 quando cultivado em OTCs.<br \/>\n<strong>Palavras-chave<\/strong>: 1. Rubiaceae. \u00a0\u00a02. Mudan\u00e7as clim\u00e1ticas.\u00a0\u00a0 3. CO<sub>2\u00a0 <\/sub>\u00a0elevado<\/p>\n<h4 style=\"text-align: center\"><\/h4>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<p style=\"text-align: center\"><a href=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/2018\/06\/tese-final-eunice-reis-batista-05-08-2015.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter\" src=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/2013\/09\/pdf_grande.gif\" alt=\"pdf_grande\" width=\"60\" height=\"60\" \/><\/a><span style=\"color: #000000\"><a style=\"color: #000000\" href=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/2018\/06\/tese-final-eunice-reis-batista-05-08-2015.pdf\">Eunice Reis Batista<\/a><\/span><a href=\"https:\/\/smastr16.blob.core.windows.net\/pgibt\/2018\/06\/tese-final-eunice-reis-batista-05-08-2015.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><br \/>\n<strong>Respostas fisiol\u00f3gicas e metab\u00f3licas de duas cultivares de <em>Coffea arabica<\/em> L. (Rubiaceae) submetidas a atmosferas enriquecidas em CO<sub>2<\/sub> em C\u00e2maras de Topo Aberto e sistema FACE<\/strong><br \/>\n<\/a><\/p>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n<p style=\"text-align: center\"><strong>\u00a0<a href=\"www.infraestruturameioambiente.sp.gov.br\/pgibt\/dissertacoesteses\/\">VOLTAR AS DISSERTA\u00c7\u00d5ES E TESES<\/a><\/strong><\/p>\n<hr align=\"center\" noshade=\"noshade\" size=\"1\" width=\"100%\" \/>\n","protected":false},"excerpt":{"rendered":"<p>Eunice Reis Batista No dia 25 de fevereiro de 2015, a aluna da P\u00f3s-gradua\u00e7\u00e3o em Biodiversidade Vegetal e Meio Ambiente do Instituto de Bot\u00e2nica de S\u00e3o Paulo (IBt), Eunice Reis Batista defendeu sua tese de doutorado intitulada\u00a0\u201cRespostas fisiol\u00f3gicas e metab\u00f3licas de duas cultivares de Coffea arabica L. (Rubiaceae) submetidas a atmosferas enriquecidas em CO2 em [&hellip;]<\/p>\n","protected":false},"author":63,"featured_media":0,"parent":249,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/6474"}],"collection":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/users\/63"}],"replies":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/comments?post=6474"}],"version-history":[{"count":3,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/6474\/revisions"}],"predecessor-version":[{"id":6479,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/6474\/revisions\/6479"}],"up":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/249"}],"wp:attachment":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/media?parent=6474"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}