{"id":6359,"date":"2018-04-24T11:47:19","date_gmt":"2018-04-24T14:47:19","guid":{"rendered":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/?page_id=6359"},"modified":"2018-04-24T11:48:05","modified_gmt":"2018-04-24T14:48:05","slug":"vanessa-palermo-bolsoni-ms","status":"publish","type":"page","link":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/dissertacoesteses\/vanessa-palermo-bolsoni-ms\/","title":{"rendered":"Vanessa Palermo Bolsoni MS"},"content":{"rendered":"

\"faixapos6\"<\/a><\/h2>\n
\n

Vanessa Palermo Bolsoni<\/h2>\n
\n

No dia 18 de abril de 2017, no Instituto de Bot\u00e2nica de S\u00e3o Paulo, foi realizada a defesa da disserta\u00e7\u00e3o de mestrado pela aluna Vanessa Palermo Bolsoni, intitulada \u201cCompostos org\u00e2nicos vol\u00e1teis (COV) emitidos por esp\u00e9cies vegetais da Mata Atl\u00e2ntica sob alto oz\u00f4nio\u201d. O trabalho foi avaliado e aprovado pela banca examinadora composta pela presidente e orientadora Profa. Dra. Silvia Ribeiro de Souza (N\u00facleo de Pesquisa em Ecologia, Instituto de Bot\u00e2nica) e pelos pesquisadores Prof. Dr. Gustavo Muniz Dias (Centro de Ci\u00eancias Naturais e Humanas, Universidade Federal do ABC) e Profa. Dra. Regina Maria de Moraes (N\u00facleo de Pesquisa em Ecologia, Instituto de Bot\u00e2nica).<\/p>\n

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Compostos org\u00e2nicos vol\u00e1teis (COV) emitidos por esp\u00e9cies vegetais da Mata Atl\u00e2ntica sob alto oz\u00f4nio<\/h3>\n
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ABSTRACT<\/strong><\/h4>\n

The plants emit a significant amount of volatile organic compounds (VOCs) that are very important chemically and biologically in the environment. The amount of VOC emitted under future climate change is of great concern because it affects climate and ecological interactions. In addition, the role of VOC as precursors of the photochemical processes occurring in the atmosphere is well known, being one of the main actors in the production of particles and ozone in the troposphere. Therefore, this study aimed to characterize the volatiles produced constitutively and as a result of the exposure to ozone by young plants of Croton floribundus<\/em> Spreng., Piptadenia gonoacantha<\/em> (Mart.) J.F. Macbr., and Astronium graveolens<\/em> Jacq., in order to evaluate the action of ozone in the profile and emission rate of the volatiles produced by these plant species, in order to verify if constitutive and induced volatiles are recognized as important for ecological interactions and for atmospheric photochemical processes. Seedlings of the three plant species were purchased in commercial nursery, transplanted and accommodated for a month in a greenhouse with filtered air, located at the Botany Institute of S\u00e3o Paulo-SP. The treatments were filtered air (AF) and filtered air enriched with a dose of 80 ppb of ozone for 2 (AF + 2dO3<\/sub>) and 4 (AF + 4dO3<\/sub>) days of exposure. The volatiles sampled constitutively and as a result of exposure to ozone were desorbed in nitrogen gas by thermal desorption system, Perkin Helmer Turbo matrix 650 ATD and automatically analyzed in gas chromatography coupled to the mass spectrometer (CG-MS). It was identified 25 compounds for constitutive samples and 49 compounds for those induced by ozone, including the three plant species. The constitutives were discriminated in chemotypes due to the emission of distinct compounds and markers for each species, with predominance of the monoterpene \u03b2-Mircene and the sesquiterpene Farnesane for Astronium graveolens<\/em>, the monoterpene \u03b2-ocimene and the sesquiterpene \u03b3-Elemene, in addition to the green leaf volatile 3-Hexen-1-ol for Croton floribundus, <\/em>and green leafy volatiles such as 3-hexen-1-ol and Hexanal, also the methyl salicylate for Piptadenia gonoacantha<\/em>. When submitted to exposure to ozone, plant species presented different responses. A. graveolens<\/em> and C. floribundus<\/em> responded significantly to ozone treatments, emitting compounds induced by the action of this pollutant, such as \u03b2-Mircene, Benzaldehyde, Nonanal, \u03b3-Murolene, Decanal and Geranylacetone for A. graveolens<\/em>, and the compounds Benzaldehyde, Trans-\u03b2-ionone, Decanal, 3-Hexen-1-ol (Z), Hexanal, Methyl salicylate, \u03b3-Elemene and (-) – \u03b2-Bourbonene for C. floribundus<\/em>. \u00a0P. gonocantha<\/em> did not present a defense response from the perspective of volatile compounds. The main VOCs found in the three species of plants studied are highly relevant to the environment, in addition to conferring an indicative potential of tolerance of these species to stress of abiotic origin.
\nKeywords<\/strong>: native species, volatile organic compounds, constitutives, ozone<\/p>\n

RESUMO<\/strong><\/h4>\n

As plantas emitem uma quantidade significativa de compostos org\u00e2nicos vol\u00e1teis (COV) que apresentam suma import\u00e2ncia qu\u00edmica e biol\u00f3gica no meio ambiente. A quantidade de COV emitida sob as mudan\u00e7as clim\u00e1ticas futuras \u00e9 de grande preocupa\u00e7\u00e3o por afetar o clima e as intera\u00e7\u00f5es ecol\u00f3gicas. Al\u00e9m disso, \u00e9 bem conhecido o papel dos COV como precursores dos processos fotoqu\u00edmicos ocorridos na atmosfera, sendo eles um dos principais atores na produ\u00e7\u00e3o de part\u00edculas e oz\u00f4nio na troposfera. Portanto, este estudo visou caracterizar os vol\u00e1teis produzidos constitutivamente e em decorr\u00eancia da exposi\u00e7\u00e3o ao oz\u00f4nio, por indiv\u00edduos jovens de Croton floribundus <\/em>Spreng., Piptadenia gonoacantha<\/em>\u00a0(Mart.) J.F Macbr., e Astronium graveolens<\/em> Jacq., a fim de avaliar a a\u00e7\u00e3o do oz\u00f4nio no perfil e na taxa de emiss\u00e3o dos vol\u00e1teis produzidos por essas esp\u00e9cies vegetais, visando verificar se os vol\u00e1teis constitutivos e induzidos s\u00e3o reconhecidamente importantes para as intera\u00e7\u00f5es ecol\u00f3gicas e para os processos fotoqu\u00edmicos atmosf\u00e9ricos. Mudas das tr\u00eas esp\u00e9cies vegetais foram adquiridas em viveiro comercial, transplantadas e acomodadas por um m\u00eas em casa de vegeta\u00e7\u00e3o com ar filtrado, situada no Instituto de Bot\u00e2nica de S\u00e3o Paulo-SP. Foram realizados os tratamentos ar filtrado (AF) e ar filtrado enriquecido com uma dose de 80 ppb de oz\u00f4nio durante 2 (AF+2dO3<\/sub>) e 4 (AF+4dO3<\/sub>) dias de exposi\u00e7\u00e3o. Os vol\u00e1teis amostrados constitutivamente e em decorr\u00eancia da exposi\u00e7\u00e3o foram dessorvidos em nitrog\u00eanio gasoso por sistema de dessor\u00e7\u00e3o t\u00e9rmica, Turbo matrix 650 ATD da Perkin Helmer, e automaticamente analisados em cromatografia gasosa acoplada a espectr\u00f4metro de massas (CG-MS). Foram identificados 25 compostos para as amostras constitutivas e 49 compostos para os induzidos pelo oz\u00f4nio, incluindo as tr\u00eas esp\u00e9cies vegetais. Os constitutivos foram discriminados em quimiotipos devido \u00e0 emiss\u00e3o de compostos distintos e marcadores para cada esp\u00e9cie, com predomin\u00e2ncia do monoterpeno \u03b2-Mirceno e o sesquiterpeno Farnesano para Astronium<\/em> graveolens<\/em>, o monoterpeno \u03b2-ocimeno e o sesquiterpeno \u03b3-Elemeno, al\u00e9m do vol\u00e1til de folhas verdes 3-Hexen-1-ol para Croton floribundus<\/em>, e os vol\u00e1teis de folhas verdes como 3-hexen-1-ol e Hexanal, ainda o Metil salicilato para Piptadenia gonoacantha<\/em>. Quando submetidas \u00e0 exposi\u00e7\u00e3o ao oz\u00f4nio, as esp\u00e9cies vegetais apresentaram respostas diferenciadas. A. graveolens<\/em> e C. floribundus<\/em> responderam de forma significativa aos tratamentos com oz\u00f4nio, emitindo compostos induzidos pela a\u00e7\u00e3o desse poluente, como por exemplo o \u03b2-Mirceno, Benzalde\u00eddo, Nonanal, \u03b3-Muroleno, Decanal e Geranil acetona para A. Graveolens, <\/em>e os compostos Benzalde\u00eddo, Trans-\u03b2-Ionone, Decanal, 3-Hexen-1-ol (Z), Hexanal, Metil salicilato, \u03b3-Elemeno e (-)-\u03b2-Bourboneno para C. floribundus<\/em>. J\u00e1 P. gonocantha<\/em> n\u00e3o apresentou uma resposta de defesa sob a perspectiva dos compostos vol\u00e1teis. Os principais COV encontrados nas tr\u00eas esp\u00e9cies vegetais em estudo apresentam alta relev\u00e2ncia ecol\u00f3gica, al\u00e9m de conferirem potencial indicativo de toler\u00e2ncia destas esp\u00e9cies frente ao estresse de origem abi\u00f3tica.
\nPalavras-chave<\/strong>: esp\u00e9cies nativas, compostos org\u00e2nicos vol\u00e1teis, constitutivos, oz\u00f4nio<\/p>\n

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\"pdf_grande\"<\/a>Vanessa Palermo Bolsoni
\n<\/a><\/span>Compostos org\u00e2nicos vol\u00e1teis (COV) emitidos por esp\u00e9cies vegetais da Mata Atl\u00e2ntica sob alto oz\u00f4nio<\/a><\/p>\n

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\u00a0VOLTAR AS DISSERTA\u00c7\u00d5ES E TESES<\/a><\/strong><\/p>\n

\n","protected":false},"excerpt":{"rendered":"

Vanessa Palermo Bolsoni No dia 18 de abril de 2017, no Instituto de Bot\u00e2nica de S\u00e3o Paulo, foi realizada a defesa da disserta\u00e7\u00e3o de mestrado pela aluna Vanessa Palermo Bolsoni, intitulada \u201cCompostos org\u00e2nicos vol\u00e1teis (COV) emitidos por esp\u00e9cies vegetais da Mata Atl\u00e2ntica sob alto oz\u00f4nio\u201d. O trabalho foi avaliado e aprovado pela banca examinadora composta […]<\/p>\n","protected":false},"author":63,"featured_media":0,"parent":249,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/6359"}],"collection":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/users\/63"}],"replies":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/comments?post=6359"}],"version-history":[{"count":1,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/6359\/revisions"}],"predecessor-version":[{"id":6361,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/6359\/revisions\/6361"}],"up":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/249"}],"wp:attachment":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/media?parent=6359"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}