{"id":5850,"date":"2017-03-31T16:38:20","date_gmt":"2017-03-31T19:38:20","guid":{"rendered":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/?page_id=5850"},"modified":"2018-05-07T12:10:11","modified_gmt":"2018-05-07T15:10:11","slug":"2016-sarah-cristina-oliveira-rocha-ms","status":"publish","type":"page","link":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/dissertacoesteses\/2016-sarah-cristina-oliveira-rocha-ms\/","title":{"rendered":"Sarah Cristina Oliveira Rocha MS"},"content":{"rendered":"

\"faixapos6\"<\/a><\/h2>\n
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Sarah Cristina Oliveira Rocha<\/h2>\n
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Em 14 de mar\u00e7o de 2016, no anfiteatro do Instituto de Bot\u00e2nica (IBt), a aluna da p\u00f3s-gradua\u00e7\u00e3o em Biodiversidade Vegetal e Meio Ambiente do Instituto de Bot\u00e2nica de S\u00e3o Paulo (IBt), Sarah Cristina Oliveira Rocha (bolsista FAPESP) defendeu sua disserta\u00e7\u00e3o de mestrado intitulada\u00a0\u201cCaracteriza\u00e7\u00e3o morfol\u00f3gica e molecular de esp\u00e9cies de Leptolegniaceae e Saprolegniaceae de \u00e1reas de Mata Atl\u00e2ntica do estado de S\u00e3o Paulo\u201d.<\/p>\n

A banca examinadora foi composta pela sua orientadora Dra. Carmen Lidia Amorim Pires-Zottarelli (orientadora\/Instituto de Bot\u00e2nica), Dra. Christiane Ceriani Apparecido (Instituto Biol\u00f3gico) e Dra. Iracema Helena Schoenlein Crusius (Instituto de Bot\u00e2nica).<\/p>\n

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Caracteriza\u00e7\u00e3o morfol\u00f3gica e molecular de esp\u00e9cies de Leptolegniaceae e Saprolegniaceae de \u00e1reas de Mata Atl\u00e2ntica do estado de S\u00e3o Paulo<\/h3>\n
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ABSTRACT<\/strong><\/h4>\n

Saprolegniaceae sensu lato<\/em> (Order Saprolegniales, Kingdom Straminipila) was divided in 1999 by M.W. Dick and collaborators in two families, Saprolegniaceae and Leptolegniaceae considering the morphological and molecular data (SSU rDNA region) of some representatives. These two families consist of saprotrophic and parasitic species typically found in terrestrial and aquatic ecosystems performing important ecological roles. However, both families are scarcely studied being necessary more studies in order to understand the phylogenetic relationships between its genera. Considering this, the aim of this study was to characterize through the morphological and molecular data the specimens of Leptolegniaceae and Saprolegniaceae, in order to contribute to the knowledge of the group. For this, 45 specimens were selected, being the majority collected at the Atlantic Forest of S\u00e3o Paulo State, representing 31 species belonging to the genus Achlya,<\/em> Aphanomyces<\/em>, Aplanopsis<\/em>, Brevilegnia<\/em>, Dictyuchus<\/em>, Leptolegnia<\/em>, Phragmosporangium<\/em>, Plectospira<\/em>, Pythiopsis<\/em> and Saprolegnia<\/em>. All specimens were characterized according to the morphology and\/or molecular data (LSU and ITS rDNA regions) in order to confirm previous morphological identification and documented by photographs. From the species studied, the identity of 26 were confirmed, with the sequences obtained deposited in GenBank and used for phylogenetic analysis. The results corroborate a recent study by G.W. Beakes and collaborators in 2014, which rejects Leptolegniaceae and accepted it into the order Saprolegniales together with Saprolegniaceae, Verrucalvaceae and Achlyaceae (proposed by the authors). From the species studied, eight were sequenced for the first time in this study (Achlya orion <\/em>Coker & Couch, A. oviparvula <\/em>A.L. Rogers & Beneke, Brevilegnia longicaulis <\/em>T.W. Johnson, Dictyuchus pseudodictyon <\/em>Coker & Braxton, Leptolegnia eccentrica <\/em>Coker, Phragmosporangium uniseriatum <\/em>R.L. Seym., Pythiopsis irregularis<\/em> R.L. Seym.\u00a0 <\/em>and Saprolegnia truncata <\/em>R.L. Seym.). The phylogenetic analyses have shown that the genus Leptolegnia<\/em> belongs to Saprolegniaceae, however this is not a monophyletic genus, considering the separation of L. eccentrica <\/em>from the other representatives. It was observed that the genus Phragmosporangium <\/em>belongs to Verrucalvaceae, and not to Saprolegniaceae, as proposed in the original description of the genus\/species. For the first time Brazilian specimens are included into phylogenetic analysis, which contributed to the discovery of a new species of Saprolegnia<\/em>. This study makes a significant contribution to the taxonomy of Saprolegniales and demonstrates the importance of the morphological and molecular studies.
\nKeywords<\/strong>: Saprolegniales, ITS and LSU rDNA, Oomycota, Taxonomy<\/p>\n

RESUMO<\/strong><\/h4>\n

Saprolegniaceae sensu lato<\/em> (Ordem Saprolegniales, Reino Straminipila) foi dividida em 1999 por M.W. Dick e colaboradores em duas fam\u00edlias, Saprolegniaceae e Leptolegniaceae, considerando dados morfol\u00f3gicos e moleculares (regi\u00e3o SSU do rDNA) de alguns de seus representantes. Estas fam\u00edlias s\u00e3o compostas por esp\u00e9cies sapr\u00f3bias e parasitas, normalmente presentes nos ecossistemas terrestres e aqu\u00e1ticos, onde desempenham importantes fun\u00e7\u00f5es ecol\u00f3gicas. No entanto, s\u00e3o fam\u00edlias pouco estudadas e que necessitam de um conhecimento mais aprofundado, visando o entendimento das rela\u00e7\u00f5es filogen\u00e9ticas entre os g\u00eaneros. Em vista disto, o principal objetivo deste estudo foi a caracteriza\u00e7\u00e3o morfol\u00f3gica e molecular de esp\u00e9cimes de Leptolegniaceae e Saprolegniaceae, com o intuito de contribuir para o conhecimento do grupo. Para isto, foram selecionados 45 esp\u00e9cimes, com a maioria proveniente de \u00e1reas de Mata Atl\u00e2ntica do Estado de S\u00e3o Paulo, os quais representaram 31 esp\u00e9cies pertencentes aos g\u00eaneros Achlya<\/em>, Aphanomyces<\/em>, Aplanopsis,<\/em> Brevilegnia<\/em>, Dictyuchus<\/em>, Leptolegnia<\/em>, Phragmosporangium<\/em>, Plectospira<\/em>, Pythiopsis<\/em> e Saprolegnia<\/em>. Todos os esp\u00e9cimes foram caracterizados em termos morfol\u00f3gicos e\/ou moleculares (regi\u00f5es LSU e ITS do rDNA) visando confirma\u00e7\u00e3o de identifica\u00e7\u00e3o morfol\u00f3gica pr\u00e9via, bem como documentados por meio de fotografias. Das esp\u00e9cies estudadas, 26 tiveram a identifica\u00e7\u00e3o confirmada, com as sequ\u00eancias obtidas disponibilizadas no GenBank e utilizadas para an\u00e1lises filogen\u00e9ticas. Os resultados obtidos corroboraram recente estudo realizado por G.W. Beakes e colaboradores em 2014, o qual rejeita Leptolegniaceae e aceita dentro da ordem Saprolegniales as fam\u00edlias Saprolegniaceae, Verrucalvaceae e Achlyaceae, sendo esta \u00faltima proposta por estes autores. Das esp\u00e9cies estudadas, oito foram sequenciadas pela primeira vez neste estudo, sendo elas Achlya orion <\/em>Coker & Couch, A. oviparvula <\/em>A.L. Rogers & Beneke, Brevilegnia longicaulis <\/em>T.W. Johnson, Dictyuchus pseudodictyon <\/em>Coker & Braxton, Leptolegnia eccentrica <\/em>Coker, Phragmosporangium uniseriatum <\/em>R.L. Seym., Pythiopsis irregularis<\/em> R.L. Seym.\u00a0 <\/em>e Saprolegnia truncata <\/em>R.L. Seym. As an\u00e1lises filogen\u00e9ticas realizadas comprovaram que o g\u00eanero Leptolegnia<\/em> pertence \u00e0 Saprolegniaceae, por\u00e9m este n\u00e3o \u00e9 monofil\u00e9tico, com a separa\u00e7\u00e3o de L. eccentrica<\/em> de outros representantes do g\u00eanero. Por meio deste estudo foi tamb\u00e9m poss\u00edvel constatar que o g\u00eanero Phragmosporangium<\/em> pertence \u00e0 Verrucalvaceae, e n\u00e3o mais a Saprolegniaceae, como proposto na descri\u00e7\u00e3o original do g\u00eanero\/esp\u00e9cie. Pela primeira vez esp\u00e9cimes coletados no Brasil s\u00e3o inseridos em an\u00e1lises filogen\u00e9ticas, o que permitiu a descoberta de uma nova esp\u00e9cie do g\u00eanero Saprolegnia<\/em> para a ci\u00eancia.<\/em> Este estudo contribuiu de forma significativa para a taxonomia de Saprolegniales e demonstra a import\u00e2ncia da jun\u00e7\u00e3o dos estudos morfol\u00f3gicos e moleculares.
\nPalavras-chave<\/strong>: Saprolegniales, ITS e LSU do r DNA, Oomycota, Taxonomia<\/p>\n

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\"pdf_grande\"<\/a>Sarah Cristina Oliveira Rocha
\nCaracteriza\u00e7\u00e3o morfol\u00f3gica e molecular de esp\u00e9cies de Leptolegniaceae e Saprolegniaceae de \u00e1reas de Mata Atl\u00e2ntica do estado de S\u00e3o Paulo
\n<\/a><\/p>\n

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\u00a0VOLTAR AS DISSERTA\u00c7\u00d5ES E TESES<\/a><\/strong><\/p>\n

\n","protected":false},"excerpt":{"rendered":"

Sarah Cristina Oliveira Rocha Em 14 de mar\u00e7o de 2016, no anfiteatro do Instituto de Bot\u00e2nica (IBt), a aluna da p\u00f3s-gradua\u00e7\u00e3o em Biodiversidade Vegetal e Meio Ambiente do Instituto de Bot\u00e2nica de S\u00e3o Paulo (IBt), Sarah Cristina Oliveira Rocha (bolsista FAPESP) defendeu sua disserta\u00e7\u00e3o de mestrado intitulada\u00a0\u201cCaracteriza\u00e7\u00e3o morfol\u00f3gica e molecular de esp\u00e9cies de Leptolegniaceae e […]<\/p>\n","protected":false},"author":63,"featured_media":0,"parent":249,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/5850"}],"collection":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/users\/63"}],"replies":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/comments?post=5850"}],"version-history":[{"count":2,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/5850\/revisions"}],"predecessor-version":[{"id":6405,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/5850\/revisions\/6405"}],"up":[{"embeddable":true,"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/pages\/249"}],"wp:attachment":[{"href":"https:\/\/www.infraestruturameioambiente.sp.gov.br\/pgibt\/wp-json\/wp\/v2\/media?parent=5850"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}